Neogen ELISA Drug Detection Kit11β-Prostaglandin F (11β-PGF) is derived from PGD2 by the action of 11-ketoreductase. It is a potent bronchoconstrictor agent. It also causes hypertension in animals. Plasma levels of 11β-PGF are highly elevated in patients with systemic mastocytosis with an overproduction of PGD2.

This is an ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative analysis of 11β-Prostaglandin F levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 11β-PGF in the sample for a limited number of binding sites on the antibody coated plate.

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
11β-Prostaglandin F2α 100.00%
Prostaglandin F2α 0.24%
Prostaglandin E2 0.21%
Thromboxane B2 0.21%
Prostaglandin D2 0.01%
Leukotriene B4 <0.01%
Prostaglandin A1 <0.01%
Prostaglandin A2 <0.01%
Prostaglandin B1 <0.01%
Prostaglandin B2 <0.01%
Prostaglandin F1α <0.01%
6-keto-Prostaglandin E1 <0.01%
6-keto-Prostaglandin F1α <0.01%
13,14-dihydro-15-keto-Prostaglandin F2α <0.01%
15-keto-Prostaglandin F2α <0.01%
11-dehydro-Thromboxane B2 <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.015 ng/mL
   50% B/B0 = 0.05 ng/mL
Assay Range: 0.01 - 1.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
404410* 11β-Prostaglandin F, 96 well kit 

 

* Minimum lead times and order quantities required, contact Neogen for more details.

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

For research use only.