Neogen ELISA Drug Detection Kit13,14-dihydro-15-keto-Prostaglandin F (13,14-dihydro-15-keto-PGF) is a major metabolite of PGF in tissues. It is derived from PGF through the consecutive actions of 15-hydroxy Prostaglandin dehydrogenase and Prostaglandin Δ13-reductase, because of the rapid metabolism of PGF, quantitation of 13,14-dihydro-15-keto-PGF in circulation can be a good index for PGF synthesis in tissue.

This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of 13,14-dihydro-15-keto-Prostaglandin F levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 13,14-dihydro-15-keto-PGF in the sample for a limited number of binding sites on the antibody coated plate.

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
13,14-dihydro-15-keto-Prostaglandin F 100.00%
15-keto-dihydro-Prostaglandin E2 1.00%
15-keto-Prostaglandin F 0.13%
Leukotriene B4 <0.01%
Prostaglandin A1
<0.01%
Prostaglandin A2 <0.01%
Prostaglandin B1 <0.01%
Prostaglandin B2 <0.01%
Prostaglandin D2 <0.01%
Prostaglandin E2
<0.01%
Prostaglandin F <0.01%
Prostaglandin F <0.01%
6-keto-Prostaglandin E1 <0.01%
6-keto-Prostaglandin F <0.01%
15-keto-Prostaglandin E2 <0.01%
Thromboxane B2 <0.01%
11-dehydro-Thromboxane B2 <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.04 ng/mL
   50% B/B0 = 0.23 ng/mL
Assay Range: 0.02 - 2.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
404610* 13, 14-dihydro-15-keto-Prostaglandin F (alpha) Kit, 96 well kit 

 

* Minimum lead times and order quantities required, contact Neogen for more details.

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

For research use only.