Neogen ELISA Drug Detection KitIsoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of a representative isoprostane, 15-isoprostane F2t (also known as 8-epi-PGF or 8-iso-PGF or 8-isoprostane). Levels of 15-isoprostane F2t in biological fluids have been shown to be useful for assessment of oxidant stress in vivo. 15-isoprostane F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis and carcinogenesis.

This kit can be used for the quantification of free 15-isoprostane F2t in urine, plasma, serum or tissue samples following solid phase extraction of the isoprostane-containing fraction. Instructions are also provided for the quantification of total 15-isoprostane F2t following hydrolysis of phospholipids.

NOTE: Alternatively, urine samples may be analyzed without solid phase extraction using a separate kit (Product #430110) available from Neogen Corporation.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
15-Isoprostane F2t 100%
9α,11β-Prostaglandin F 4.1%
13,14-dihydro-15-keto-PGF 3%
9β,11α-Prostaglandin F <0.01%
Prostaglandin F <0.01%
6-keto-Prostaglandin F <0.01%
Prostaglandin E2 <0.01%
Prostaglandin D2 <0.01%
Arachidonic Acid <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.03 ng/mL
   50% B/B0 = 5.0 ng/mL
Assay Range: 0.05 - 100 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 100 µL
Total Assay Incubation Time: 2.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal

Order Details

Product # Product Description
430010 15-Isoprostane F2t, 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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