Neogen ELISA Drug Detection KitAndrostenedione is synthesized in the adrenal gland. It is the primary precursor of testosterone in women. Therefore, in women, androstenedione contributes significantly to the level of androgenic activity, while in men this contribution is nil.

Quantitation of androstenedione may indicate the androgenic activity in women.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Androstenedione 100%
Estrone 1.5%
Pregnenolone 0.20%
Deoxycorticosterone 0.16%
Estrone-3-Sulfate 0.16%
17β-Estradiol 0.08%
Hydrocortisone 0.08%
Prednisolone 0.08%
Estriol 0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.015 ng/mL
   50% B/B0 = 0.08 ng/mL
Assay Range: 0.01 - 1ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
402010 Androstenedione, 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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Follow the simple step-by-step guide to generate your data.

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