Activity of Neogen's TMB Substrates
* Alert K-Blue Aqueous has similar activity to K-Blue Aqueous

Neogen recognizes that one TMB microwell substrate formulation will not meet the specifications of all HRP-based immunoassays. Therefore, Neogen offers multiple unique one-bottle TMB microwell colorimetric substrate formulations to meet specific requirements for different assay systems.

All of Neogen’s TMB microwell substrate formulations are one-bottle stabilized chromogenic substrates for use with horseradish peroxidase immunoassays. The formulations contain 3,3',5,5' TMB and hydrogen peroxide (H2O2) in a ready-to-use format with long term stability. Our TMB substrates turn a deep blue color in the presence of peroxidase labeled conjugate. These substrates are not applicable for use with assays requiring a precipitating substrate. Neogen substrates have common characteristics of low background and excellent lot-to-lot consistency.

Available Products

 

Microwell HRP Substrates

ProductActivityShelf LifeProduct FeaturesAvailable Sizes
Enhanced K-Blue High activity
See above chart for kinetics
48 months when stored at 4°C
  • Longest shelf-life of Neogen TMB substrates
  • RTU (ready-to-use)
  • Low background
  • High consistency betweenlots
  • 200 mL
  • 500 mL
  • 1 L
  • 20 L (1 x 20 L)
K-Blue Aqueous High activity: Kinetics similar to Enhanced K-Blue
See above chart for kinetics
36 months when stored at 4°C
  • 100% solvent free
  • Ideal when working under strict regulatory requirements
  • RTU (ready-to-use)
  • Low background
  • High consistency between lots
  • 200 mL
  • 500 mL
  • 1 L
  • 20 L (1 x 20 L)
Alert K-Blue Aqueous High activity: Kinetics similar to K-Blue Aqueous 36 months when stored at 4°C
  • Clearly indicates when substrate and acid stop have been added to the wells (turns pink)
  • Ideal for sandwich-based HRP assays
  • 100% solvent free
  • RTU (ready-to-use)
  • Low background
  • High consistency between lots
  • 200 mL
  • 500 mL
  • 1 L
  • 20 L (1 x 20 L)
Custom TMB Lower activity: 20–30% Reduced activity of Enhanced K-Blue
See above chart for kinetics
36 months when stored at 4°C
  • Ideal for assays requiring a less active substrate
  • RTU (ready-to-use)
  • Low background
  • High consistency between lots
  • 200 mL
  • 500 mL
  • 1 L
  • 20 L (1 x 20 L)

For research and manufacturing use only.