Neogen ELISA Drug Detection KitCorticosterone is a glucocorticoid secreted by the adrenal cortex in response to stress. Glucocorticoids are also essential for proper metabolism of fats, proteins, and carbohydrates in the body. While cortisol and corticosterone are both produced in response to stress in humans, corticosterone is the predominant glucocorticoid produced in mice and rats. Corticosterone has also been used as a predictor of stress in a variety of wild animals. In particular corticosterone levels were increased in Galapagos marine iguanas under famine conditions brought on by El Niño.

While not always predictive of environmental stress in the wild, conditions such as starvation, rapid temperature change, or changes in daily routine can cause increased corticosterone levels in laboratory mice and rats.

Corticosterone is also administered to treat inflammation because like many other steroids, it is a powerful anti-inflammatory agent.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Corticosterone 100%
Deoxycorticosterone 38%
6-Hydroxycorticosterone 19%
Progesterone 5.1%
Tetrahydrocorticosterone 2.7%
Prednisolone 1.5%
Cortisol 1.1%
Pregnenolone 0.85%
11-Epicorticosterone 0.78%
Cortisone 0.27%
21-Desoxycortisol 0.27%
d-Aldosterone 0.13%
Testosterone 0.12%
17α-Hydroxyprogesterone 0.12%
Prednisone 0.10%
Dexamethasone 0.03%
Cholesterol <0.01%
Estradiol <0.01%
Estriol <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.05 ng/mL
   50% B/B0 = 0.37 ng/mL
Assay Range: 0.05 - 5 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
402810 Corticosterone Kit, 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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Follow the simple step-by-step guide to generate your data.

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Calculate Results

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