This is a simple, inexpensive and reliable assay for the quantitative analysis of creatinine in biological fluids. This assay is useful for normalizing isoprostane levels in human urine samples.
Creatine (Cr) produced in the kidney, liver and pancreas is transported to the brain and muscle and phosphorylated. However, a small proportion of free Cr is converted irreversibly to Creatinine (Crn) in the muscular tissue in proportion to the muscle mass. The amount of Crn excreted daily by an individual is relatively constant. Thus, urinary creatinine levels may be used as an index of standardization. Also, since the rate of nonenzymatic production of Crn from Cr is nearly constant and because >90% of the total bodily Cr is found in muscle tissue, 24-h urinary Crn excretion is used as an estimate of total muscle mass. Normal urinary creatinine values for men and women range from 9.7 – 24.7 and 7.9 – 14.2 mmol/24h respectively. Changes in excretion rate may be indicative of impaired renal metabolism.
Standard Curve in EIA Buffer
|Standard Range: 0 mg/mL - 10 mg/mL
Number of Samples: 44 in duplicate
Assay Sample Size: 25 µL
Total Assay Incubation Time: 15 minutes
Wavelength: 490/500 nm
Storage Conditions: 4°C for standard RT for all other components
|Product #||Product Description|
|430210||Creatinine Kit, 96 well Kit|
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
Calculation of Results
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