Neogen ELISA Drug Detection KitAdenosine 3':5'-cyclic monophosphate (cyclic AMP, cAMP) is one of the most important secondary messengers involved in signal transduction, gene regulation, steroid hormone biosynthesis and intracellular signaling. Its regulatory role extends to the nervous system, cell growth and differentiation, immune mechanisms and general metabolism. Cyclic AMP production is stimulated by compounds (hormones, drugs, neuromodulators) binding to target cells through cell membrane receptors. These binding events activate G-proteins which in turn activate the enzyme adenylate cyclase. Adenylate cyclase converts ATP to cyclic AMP. Cyclic AMP-dependent protein kinases can then be activated resulting in phosphorylation of substrate proteins. Cyclic AMP levels may aid in understanding the mechanism of action of a variety of hormones and biologically active substances.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Cyclic AMP 100%
Cyclic GMP 0.07%
Adenosine 0.01%
Adenine 0.01%
AMP 0.01%
ADP 0.01%
ATP 0.01%
Guanine 0.01%
GMP 0.01%
GTP 0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.03 ng/mL
   50% B/B0 = 0.10 ng/mL
Assay Range: 0.02 - 2.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: -20°C for lyophilized conjugate, 4°C for all other kit components
Antibody: Polyclonal, Goat

Order Details

Product # Product Description
403110* Cyclic AMP Kit, 96 well Kit

* Minimum lead times and order quantities required, contact Neogen for more details.

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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