Neogen ELISA Drug Detection Kit17β-Estradiol is considered the true ovarian hormone in women. It is the most potent estrogen, including Estrone and Estriol. 17β-Estradiol is responsible for the reproductive epithelia, breast growth, maturation of long bones, and development of the secondary female sex characteristics. The 17β-Estradiol level in plasma is used in assaying for fertility, amenorrhea and precocious puberty in girls.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
17β-Estradiol 100%
Testosterone 1%
Estriol 0.41%
Estrone 0.10%
Dehydroepiandrosterone 0.03%
Aldosterone <0.02%
Androstenedione <0.02%
Corticosterone <0.02%
Cortisol <0.02%
Cortisone <0.02%
Deoxycorticosterone <0.02%
17-Hydroxyprogesterone <0.02%
Pregnenolone <0.02%
Progesterone <0.02%
17α-Ethynylestradiol <0.01%
β-Estradiol 17-(β-D-glucuronide) <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.03 ng/mL
   50% B/B0 = 0.28 ng/mL
Assay Range: 0.02 - 2.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions:  4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
402110 Estradiol Kit, 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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