Neogen ELISA Drug Detection KitEstriol is a female hormone that exists in blood in the free and conjugated forms (primarily estriol-3-sulfate-16-glucuronide, estriol-16-glucuronide and estriol-3-sulfate). Measurement of the total estriol has been shown to be useful in monitoring the fetal function. During pregnancy, virtually all of the estriol in the maternal circulation is synthesized in the feto-placental unit. Estriol begins to appear in the maternal circulatory system as a result of synthesis by the feto-placental unit in the ninth week of gestation. During the third trimester, estriol levels in serum and urine normally increase rapidly. Therefore, a low or declining estriol level may be indicative of fetal distress. Although a single estriol measurement may have some value as a serum test, serial determinations to monitor estriol are considered more useful.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Estriol 100%
17β-Estradiol 2%
Estrone 2%
Androsterone 0.02%
Estrone-3-Sulfate 0.02%
Testosterone 0.02%
Androstenedione <0.01%
Dehydroisoandrosterone <0.01%
Deoxycorticosterone <0.01%
Hydrocortisone <0.01%
Prednisolone <0.01%
Pregnenolone <0.01%
Progesterone <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.08 ng/mL
   50% B/B0 = 0.38 ng/mL
Assay Range: 0.04 - 4 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
402610* Estriol Kit, 96 well kit

* Minimum lead times and order quantities required, contact Neogen for more details.

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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