Neogen ELISA Drug Detection KitLeukotriene B4 (LTB4) is derived from LTA4 which in turn is biosynthesized from arachidonic acid. LTB4 is produced in phagocytic cells such as leukocytes and macrophages. It is the most potent chemotactic substance ever described for leukocytes. It also stimulates vascular permeability and may play a significant role in mediating inflammatory responses. The availability of an ELISA for LTB4 will facilitate the evaluation of the physiological and pathological roles of this lipid mediator.

This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of Leukotriene B4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Leukotriene B4 in the sample for a limited number of binding sites.

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Leukotriene B4 100.00%
6-trans-LTB4 25.00%
Leukotriene B5 14.60%
5(S), 12(s) DIHETE 6.00%
Leukotriene D4 0.96%
20-Hydroxy-LTB4 0.50%
Leukotriene E4 0.30%
Leukotriene C4 0.20%
5(S)HETE 0.15%
20-Carboxy-LTB4 <0.10%
Arachidonic Acid <0.10%
12(S)HETE <0.10%
12(R)HETE <0.10%
15-HETE <0.01%
Prostaglandin A2 <0.01%
Prostaglandin B2 <0.01%
Prostaglandin D2 <0.01%
Prostaglandin E2 <0.01%
Prostaglandin F <0.01%
6-keto-Prostaglandin F <0.01%
Thromboxane B2 <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.1 ng/mL
   50% B/B0 = 0.62 ng/mL
Assay Range: 0.04 - 4.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: -20°C for lyophilized conjugate, 4°C for all other components
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
406110 Leukotriene B4, 96 well kit 

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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