Neogen ELISA Drug Detection KitLeukotriene C4 (LTC4) is a potent mediator of immediate hypersensitivity, bronchoconstriction, smooth muscle contraction, increased vascular permeability and epithelial mucous secretion. It is derived from arachidonic acid through the 5-lipoxygenase pathway. Arachidonic acid is first converted to 5-hydroperoxy derivative which is then transformed to Leukotriene A4 (LTA4). Enzymatic addition of glutathione to LTA4 produces LTC4.

This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of Leukotriene C4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Leukotriene C4 in the sample for a limited number of binding sites.

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Leukotriene C4 100.00%
Leukotriene D4 14.00%
Leukotriene E4 7.80%
Leukotriene B4 <0.01%
Prostaglandin A2 <0.01%
Prostaglandin B2 <0.01%
Prostaglandin D2 <0.01%
Prostaglandin E2 <0.01%
Prostaglandin F <0.01%
6-keto-Prostaglandin F <0.01%
5-HETE <0.01%
12-HETE <0.01%
15-HETE <0.01%
6-trans-Leukotriene B4 <0.01%
20-0H-Leukotriene B4 <0.01%
Thromboxane B2 <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.15 ng/mL
   50% B/B0 = 0.4 ng/mL
Assay Range: 0.04 - 4.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with stop solution
Storage Conditions: -20°C for lyophilized conjugate, 4°C for all other components
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
406210 Leukotriene C4, 96 well kit 

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

For research use only.