This kit employs immunoaffinity purified Nitrate Reductase enabling the measure of total nitric oxide (NO) produced for in vitro experimental systems following enzymatic conversion to nitrite.
Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem is the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase (NaR), followed by quantitation of nitrite using Griess Reagent.
In addition to providing all necessary components in a microtiter format, this kit employs affinity purified nitrate reductase and NADH, thereby circumventing some of the potential problems re-ported for nitric oxide measurement using NADPH dependent nitrate reductases.
Standard Curve in EIA Buffer
Assay Range: 0.5 - 100 µM
|Product #||Product Description|
|430410||Nitric Oxide Kit, 96 well kit|
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
For research use only.