Neogen ELISA Drug Detection KitProstaglandin F (PGF) is derived from PGH2 which in turn is synthesized from arachidonic acid through the cyclooxygenase pathway. It is produced in various tissues. It contracts smooth muscle and has been used to induce labor. It has a luteolytic effect and has been considered a luteolytic hormone.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Prostaglandin F 100%
Prostaglandin F1 90%
Thromboxane B2 3.83%
6-keto-Prostaglandin F1 3.05%
2, 3-dinor-6-keto-Prostaglandin F 0.82%
11β-Prostaglandin F 0.39%
8-iso-Prostaglandin F 0.24%
15-keto-Prostaglandin F2 0.20%
13,14-dihydro-15-keto-Prostaglandin F 0.05%
Prostaglandin D2 0.05%
Prostaglandin E1 0.01%
11-dehydro-Thromboxane B2 <0.01%
6-keto-Prostaglandin E1 <0.01%
Leukotriene B4 <0.01%
Prostaglandin A1 <0.01%
Prostaglandin A2 <0.01%
Prostaglandin B1 <0.01%
Prostaglandin B2 <0.01%
Prostaglandin E2 <0.01%
Prostaglandin E3 <0.01%
Tetranor PGFM <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.008 ng/mL
   50% B/B0 = 0.07 ng/mL
Assay Range: 0.002 - 1.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Sheep

Order Details

Product # Product Description
404710 Prostaglandin F, 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

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