Neogen ELISA Drug Detection Kit11-d-Thromboxane B2 (11-d-TXB2) is derived from TXB2 by the action of 11-dehydrogenase. It is one of the major metabolites of TXB2 in plasma and urine. Measurement of 11-d-TXB2 in plasma represents an approach to the assessment of thromboxane formation in the human circulation.

This is an ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative analysis of 11-d-Thromboxane B2 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 11-d-TXB2 in the sample for a limited number of binding sites on the antibody coated plate.

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
11-d-Thromboxane B2 100.00%
11-d-Thromboxane B3 42.00%
11-d-2,3-dinor-Thromboxane B2 24.00%
Prostaglandin D2 0.9%
2,3-dinor-Thromboxane B2 0.5%
11β-Prostaglandin F2α 0.3%
Thromboxane B2 0.08%
Prostaglandin A2
0.08%
Prostaglandin A1 0.05%
Leukotriene B4 0.04%
Leukotriene C4 0.04%
Prostaglandin B2 0.03%
Prostaglandin E2 0.03%
Thromboxane B3 0.03%
Prostaglandin B 0.02%
Prostaglandin E1 0.02%
2,3-dinor-6-keto-prostaglandin F1α 0.02%
6-keto-Prostaglandin F1α 0.02%
13,14-dihydro-15-keto-Prostaglandin F2α <0.01%
15-keto-Prostaglandin F2α <0.01%
6-keto-Prostaglandin E1 <0.01%
Leukotriene E4 <0.01%
Prostaglandin F1α <0.01%
Prostaglandin F2α <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.3 ng/mL
   50% B/B0 = 1.5 ng/mL
Assay Range: 0.2 - 20.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C for all other components
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
408010* 11-dehydro-Thromboxane B2 Kit, 96 well kit 

* Minimum lead times and order quantities required, contact Neogen for more details.

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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