6-keto-Prostaglandin F1α (6-k-PGF1α) is a stable hydrolysed product of unstable PGI2 (prostacyclin). PGI2 is derived from PGH2 which in turn is synthesised from the cyclooxygenase pathway. Through this pathway, it is the major product produced by the endothelial cells, although it is also synthesised in other cell types such as macrophages. Prostacyclin inhibits platelet aggregation and induces vasodilation. Quantitation of prostacyclin production can be made by determining the level of 6-k-PGF1α.
This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of 6-keto-Prostaglandin F1α levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 6-k-PGF1α in the sample for a limited number of binding sites on the antibody coated plate.
Standard Curve in EIA Buffer
|Product #||Product Description|
|404310||6-keto-Prostaglandin F1α (alpha), 96 well kit|
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
Calculation of Results
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