Activity of Neogen's TMB Substrates

Enhanced K-Blue® is a one bottle stabilised chromogenic substrate for use with horseradish peroxidase immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one bottle format with long term stability and high sensitivity. Enhanced K-Blue develops a deep blue colour in the presence of peroxidase labelled conjugate, and is not applicable for use with assays requiring a precipitating substrate. This formulation does not contain DMF or DMSO.

Sensitivity: Enhanced K-Blue is a highly sensitive substrate with kinetic reactions equivalent to Neogen’s original K-Blue substrate formulation. This formulation offers the advantage of containing no DMF or DMSO and has very low background.

Stability and Storage: Enhanced K-Blue is stable for a minimum of 48 months when stored at 4°C.

Appearance: Clear to faint blue solution

Custom Packaging Service: Neogen can package Enhanced K-Blue in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen representative.

Order Details

Product # Product Description
308175 Enhanced K-Blue TMB Substrate, 200 mL
308176 Enhanced K-Blue TMB Substrate, 500 mL
308177 Enhanced K-Blue TMB Substrate, 1 L
308257 Enhanced K-Blue TMB Substrate, 20 L (1 x 20 L)

For research and manufacturing use only.

Please check with your local representative for availability of these products within your region.