Neogen ELISA Drug Detection KitLeukotriene C4/D4/E4 (LTC4/D4/E4) are potent mediators of immediate hypersensitivity, bronchoconstriction, smooth muscle contraction, increased vascular permeability and epithelial mucous secretion. They are derived from arachidonic acid through the 5-lipoxygenase pathway. The monoclonal antisera in this kit recognises LTC4, LTD4 and LTE4 for a combined quantitative value.

This is an ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative analysis of Leukotriene C4/D4/E4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and Leukotriene C4/D4/E4 in the sample for a limited number of binding sites.

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Leukotriene C4 100.00%
Leukotriene D4 80.00%
Leukotriene E4 80.00%
Leukotriene A4 2.00%
Leukotriene B4 <1.0%
Prostaglandin B2 <0.01%
Prostaglandin D2 <0.01%
Prostaglandin E2 <0.01%
Prostaglandin F <0.01%
6-keto-Prostaglandin F <0.01%
5-HETE <0.01%
12-HETE <0.01%
15-HETE <0.01%
6-trans-Leukotriene B4 <0.01%
20-0H-Leukotriene B4 <0.01%
Thromboxane B2 <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.06 ng/mL
   50% B/B0 = 0.2 ng/mL
Assay Range: 0.04 - 2.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with stop solution
Storage Conditions: -20°C for lyophilised conjugate, 4°C for all other components
Antibody: Monoclonal, Rat

Order Details

Product # Product Description
406410 Leukotriene C4/D4/E4, 96 well kit 

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

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