Neogen ELISA Drug Detection KitLipoxin A4 (LXA4) is a biologically active lipoxygenase interaction product derived from arachidonic acid. Arachidonic acid is first oxygenated by 15-lipoxygenase to form 15-HETE which is then converted by 5-lipoxygenase and epoxide hydrase to generate LXA4. LXA4 stimulates leukocyte chemotaxis without aggregation and inhibits natural killer cell cytotoxicity. It also provokes contraction of parenchymal strips and stimulates microvascular changes. Recent findings indicate that LXA4 inhibits leukocyte-dependent inflammation. Determination of LXA4 levels may provide new understanding of the role of LXA4 in basic cellular reactions and in pathophysiology of inflammation and other disease processes.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Lipoxin A4 100%
15-epi-Lipoxin A4 24%
5(S), 6(R)-DiHete 5%
Lipoxin B4 1%
15-hete 0.10%
5-hete 0.10%
12-hete <0.10%
Leukotriene B4 <0.01%
Leukotriene C4 <0.01%
Leukotriene D4 <0.01%
Leukotriene E4 <0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.04 ng/mL
   50% B/B0 = 0.15 ng/mL
Assay Range: 0.02 - 2.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions:  -20°C for lyophilised conjugate, 4°C for all other kit components
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
407010 Lipoxin A4 Kit, 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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