Neogen ELISA Drug Detection KitThis kit employs immunoaffinity purified Nitrate Reductase enabling the measure of total nitric oxide (NO) produced for in vitro experimental systems following enzymatic conversion to nitrite.

Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem is the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase (NaR), followed by quantitation of nitrite using Griess Reagent.

In addition to providing all necessary components in a microtiter format, this kit employs affinity purified nitrate reductase and NADH, thereby circumventing some of the potential problems reported for nitric oxide measurement using NADPH dependent nitrate reductases.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specifications

Assay Range: 0.5 - 100 µM
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Assay Sample Size: 5 - 85 µL
Total Assay Incubation Time: 25 minutes
Wavelength: 540 nm
Storage Conditions:
   Enzyme: -20°C
   NADH: 22°C
   All other kit components: 4°C

Order Details

Product # Product Description
430410 Nitric Oxide Kit, 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

Neogen has partnered with MyAssays.com to provide an online tool for data calculations.

Follow the simple step-by-step guide to generate your data.

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