Neogen ELISA Drug Detection KitProstaglandin E2 (PGE2) is derived from PGH2 which in turn is synthesised from arachidonic acid through the cyclooxygenase pathway. Many cell types, such as epithelial cells, fibroblasts, and macrophages produce PGE2. PGE2 induces vasoactivity, modulates immune functions, regulates sleep-awake cycles and exhibits many other activities.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Prostaglandin E2 100%
Prostaglandin B1 63%
Prostaglandin E3 52%
Prostaglandin E1 50%
Prostaglandin B2 2.65%
6-keto-Prostaglandin E1 0.91%
Prostaglandin A1 0.78%
Prostaglandin A2 0.30%
Prostaglandin F 0.78%
Prostaglandin F 0.06%
Leukotriene B4 0.02%
Prostaglandin D2 0.01%
6-keto-Prostaglandin F 0.01%
13,14-dihydro-15-keto-Prostaglandin F 0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.12 ng/mL
   50% B/B0 = 0.4 ng/mL
Assay Range: 0.10 - 4 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: -20°C for lyophilised conjugate, 4°C for all other kit components
Antibody: Monoclonal, Mouse

Order Details

Product # Product Description
406510 Prostaglandin E2 (monoclonal), 96 well kit

Reference Materials

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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