Neogen®’s BCIP/NBT membrane substrates are one-bottle, ready-to-use buffered alkaline phosphatase (AP) substrates that produce an insoluble blue or purple precipitate in the presence of phosphatase. These formulas are ideal for western blot or other blot and membrane assay applications where an insoluble reaction is desired for alkaline phosphatase (AP) enzyme detection. These substrates are not recommended for microwell or immunohistochemical applications.

  • BCIP/NBT Membrane Substrate (Blue) develops an insoluble, permanent dark blue color in the presence of alkaline phosphatase enzyme.
  • BCIP/NBT Membrane Substrate (Purple) develops an insoluble, permanent dark purple color in the presence of alkaline phosphatase enzyme.

Available Products

For research and manufacturing use only.