Neogen offers an expanding line of colorimetric (chromogenic) substrates and reagents to meet our customers’ immunoassay needs. The substrates are available for use with horseradish peroxidase (HRP) and alkaline phosphatase (AP) based microwell and membrane assays.
Neogen’s colorimetric (chromogenic) substrates offer excellent sensitivity, stability and lot-to-lot consistency. These features make them ideal for inclusion in commercial immunoassay test kits or individual in-house assay projects. We also offer immunoassay reagents including a stop solution and buffers.
Horseradish Peroxidase (HRP) applications
Neogen recognises that one TMB microwell substrate formulation will not meet the specifications of all horseradish peroxidase (HRP)-based immunoassays. Therefore, Neogen offers multiple unique one-bottle TMB microwell colorimetric substrate formulations to meet specific requirements for different assay systems.
Neogen substrates have common characteristics of low background, long-term stability and excellent lot-to-lot consistency.
Alkaline Phosphatase (AP) applications
K-Gold® substrate is a one-bottle stabilised chromogenic substrate for use with alkaline phosphatase (AP)-based immunoassays. K-Gold substrate utilizes the substrate p-nitrophenyl-phosphate (PNPP), which reacts with alkaline phosphatase to produce the chromophore p-nitrophenol. Neogen’s ready-to-use K-Gold is a single-bottle formula and does not require mixing of additional ingredients or stabilizing agents. K-Gold features excellent signal-to-noise and has extremely low background over the 30-month shelf life, making this an ideal formula for assay manufacturers.
Available Products
- Colorimetric Horseradish Peroxidase (HRP) Microwell
- Colorimetric Alkaline Phosphatase (AP) Microwell
- Colorimetric Horseradish Peroxidase (HRP) Membrane
Colorimetric Horseradish Peroxidase (HRP) microwell
Product | Shelf Life | Product Features | Available Sizes | Product Numbers |
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K-Blue Advanced | 30 months when stored at 2–8°C |
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K-Blue Advanced Plus | 24 months when stored at 2–8°C |
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Enhanced K-Blue | 48 months when stored at 2–8°C |
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Custom TMB | 36 months when stored at 2–8°C |
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K-Blue Aqueous | 36 months when stored at 2–8°C |
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Alert K-Blue Aqueous | 36 months when stored at 2–8°C |
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ABTS | 36 months when stored at 4°C |
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Colorimetric Alkaline Phosphatase (AP) Microwell
Product | Shelf Life | Product Features | Available Sizes | Product Numbers |
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K-Gold | 30 months when stored at 4°C |
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K-Gold Plus | 24 months when stored at 2-8°C |
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Colorimetric Horseradish Peroxidase (HRP) Membrane
Product | Shelf Life | Product Features | Available Sizes | Product Numbers |
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TMB Membrane |
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TMB Membrane Plus |
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