Enhanced K-Blue® Substrate (TMB)

Enhanced K-Blue^® is a one bottle stabilised chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H₂O₂) Enhanced K-Blue develops a deep blue colour in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate.

Sensitivity: Enhanced K-Blue is a highly sensitive substrate with kinetic reactions equivalent to Neogen’s original K-Blue substrate formulation. This formulation offers a linear response across a wide range of HRP concentrations and has a rapid turn-over rate. Enhanced K-Blue also features high signal-to-noise and offers very low background.

Stability and Storage: Enhanced K-Blue has the longest shelf life compared to any other TMB substrate on the market and is stable for a minimum of 48 months when stored at 4°C.

Appearance: Clear to faint blue solution.

Custom Packaging Service: Neogen can package Enhanced K-Blue in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen representative.

Order Details

Reference Materials

Enhanced K-Blue Substrate, Safety Data Sheet [308175] [308176] [308177] [308257]

For research and manufacturing use only.