Progesterone is a C-21 steroid secreted by the corpus luteum and the placenta. It is an important intermediate in steroid biosynthesis in all tissues that secrete steroid hormones. Progesterone is responsible for the progestational changes in the endometrium and cyclic changes in the cervix and vagina. The plasma progesterone level is useful in investigating the luteal function and monitoring ovulation.
Standard Curve in EIA Buffer
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity |
| Progesterone | 100% |
| Deoxycorticosterone | 2.5% |
| Corticosterone | 2% |
| Pregnenolone | 2% |
| Androstenedione | 1% |
| 17-Hydroxyprogesterone | 0.40% |
| Testosterone | 0.29% |
| Cortisol | 0.20% |
| Cortisone | 0.20% |
| Dehydroepiandrosterone | 0.20% |
| Estradiol | 0.20% |
| Estriol | 0.20% |
| Estrone | 0.20% |
Specifications
| Sensitivity: 80% B/B0 = 0.35 ng/mL 50% B/B0 = 2.9 ng/mL Assay Range: 0.4 - 40.0 ng/mL Inter-Assay CV: ≤ 10% Intra-Assay CV: ≤ 10% Size: 96 well microplate Assay Sample Size: 50 µL Total Assay Incubation Time: 1.5 hours Wavelength: 650 nm, 450 nm with acid stop Storage Conditions: 4°C Antibody: Polyclonal, Rabbit |
Order Details
| Product # | Product Description |
| 402310 | Progesterone Kit, 96 well kit |
Reference Materials
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
Calculation of Results
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