Thromboxane B2 (TXB2) is a stable hydrolyzed product of unstable TXA2 which is derived from PGH2. PGH2 is synthesized from arachidonic acid through the cyclooxygenase pathway. It is a major product following platelet aggregation induced by a variety of agents such as thrombin and collagen. It is produced not only in platelets, but also in other cell types such as fibroblasts and macrophages. Quantitation of thromboxane formation can be made by determining the level of TXB2.
Standard Curve in EIA Buffer
|Arachidonic Acid||< 0.01%|
|Leukotriene B4||< 0.01%|
|Prostaglandin A2||< 0.01%|
|Prostaglandin B2||< 0.01%|
|13,14-dihydro-15-keto-Prostaglandin F2α||< 0.01%|
80% B/B0 = 0.009 ng/mL
50% B/B0 = 0.04 ng/mL
Assay Range: 0.004 - 0.4 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit
|Product #||Product Description|
|405110||Thromboxane B2 Kit, 96 well kit|
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
Calculation of Results
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