TMB Membrane Substrate is a ready to use solution. It contains 3,3’,5,5’-tetramethyl-benzidine in a mildly acidic buffer in a one bottle format. Unreacted substrate should be faint yellow to pink in appearance. TMB Membrane Substrate develops an insoluble, permanent dark blue reaction in the presence of peroxidase. This substrate is not recommended for microwell or immunohistochemical applications. It is intended for western blot, dot blots and line assays.

Sensitivity: TMB Membrane Substrate is extremely sensitive. It offers detection in the low picogram range and can rival some chemiluminescent formulas for HRP detection. This formula offers a high signal-to-noise ratio, making it resistant to fading.

Stability and Storage: TMB Membrane Substrate is stable for 2 years, from the date manufacture, when stored at room temperature (15° to 25°C) or 48 months when stored at 2° to 8°C.

Custom Packaging Service: Neogen can package TMB Membrane Substrate in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen Corporation representative.

Order Details

Product # Product Description
311247 TMB Membrane Substrate, 100 mL
311177 TMB Membrane Substrate, 1 L

Reference Materials

For research and manufacturing use only.

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