TMB Membrane Plus Substrate is a ready-to-use solution. It contains 3,3’,5,5’-tetramethyl-benzidine in a mildly acidic buffer in a one bottle format. TMB Membrane Plus Substrate develops an insoluble, permanent dark blue reaction in the presence of peroxidase. The substrate is intended for western blot, dot blots and line assays and offers comparable performance and sensitivity to some chemiluminescent substrates. This substrate is not recommended for microwell or immunohistochemical applications. Sensitivity: TMB Membrane Plus Substrate offers extremely sensitive horseradish peroxidase (HRP) detection in the low picogram range. This formula also offers an extended dynamic range making it resistant to wash-out at higher HRP concentrations. TMB Membrane Plus also features a high signal-to-noise ratio, making it resistant to fading. Stability and Storage: TMB Membrane Plus Substrate is stable for 2 years, from the date manufacture, when stored at room temperature (15° to 25°C) or 48 months when stored at 2° to 8°C. Custom Packaging Service: Neogen can package TMB Membrane Plus Substrate in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen Corporation representative. Order Details Product # Product Description 337247 TMB Membrane Plus Substrate, 100 mL 337177 TMB Membrane Plus Substrate, 1 L For research and manufacturing use only.