Strong evidence supports that isoprostanes are excellent molecular biomarkers for oxidant stress. Indeed, 15-Isoprostane-F21 is already widely recognized as the “gold standard” of oxidant status. This ELISA kit has been developed for the rapid and accurate measurement of 15-Isoprostane-F21 in urine samples. Using the specially formulated buffers and diluents provided, one can simply dilute urine samples and perform the ELISA assay. No solid phrase extraction is needed. Results obtained using this kit have been validated by GC/MS following solid phase extraction of separate aliquots and show a very high degree of correlation (r > 0.8).
NOTE: For normalization of urinary 15-isoprostane-F21 levels we recommend determination of creatinine levels. A convenient microplate creatinine kit (#430210) is available from Neogen Corporation
Standard Curve in EIA Buffer
|9α, 11β-Prostaglandin F2α||4.1%|
|9β, 11α-Prostaglandin F2α||< 0.01%|
|Prostaglandin F2α||< 0.01%|
|6-keto-Prostaglandin F1α||< 0.01%|
|Prostaglandin E2||< 0.01%|
|Prostaglandin D2||< 0.01%|
80% B/B0 = 0.08 ng/mL
50% B/B0 = 0.45 ng/mL
Assay Range: 0.0025 - 75 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 100 µL
Total Assay Incubation Time: < 2.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
|Product #||Product Description|
|430110||Urinary Isoprostane Kit, 96 well Kit|
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
Calculation of Results
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Follow the simple step-by-step guide to generate your data.
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