Neogen offers an expanding line of chemiluminescent substrates and reagents to meet our customers’ immunoassay needs. The substrates are available for use with horseradish peroxidase (HRP) and alkaline phosphatase (AP) based microwell and membrane assays.

Compared with traditional colorimetric formulations, luminol and dioxetane based formulas offer advanced benefits including significantly increased assay sensitivity allowing you to detect lower levels of target analytes. Chemiluminescent substrate also have a lower incubation temperature producing faster time to results. These features make them ideal for inclusion in commercial immunoassay test kits or individual in-house assay projects. We also offer immunoassay reagents including a stop solution and buffers.

Horseradish Peroxidase (HRP) Applications

Neogen offers multiple luminol-based chemiluminescent substrate formulations for microwell and membrane applications for the ultimate detection of horseradish peroxidase. Neogen’s substrates offer sensitivity capabilities from picogram to femtogram.

Alkaline Phosphatase (AP) Applications

Neogen also offers ultra-sensitive 1,2-dioxetane-based chemiluminescent formulations for the detection of alkaline phosphatase in microwell and membrane applications. Neogen’s substrates offer sensitivity capabilities to the attogram level.

Available Products

Chemiluminescent Horseradish Peroxidase (HRP) Microwell and Membrane

ProductShelf LifeProduct FeaturesAvailable SizesProduct Numbers
Chemiluminescent Ultra 18 months when stored at 4°C
  • Maximum sensitivity, femtogram (10-15 g)
  • Luminol-based chemistry
  • Two component system
  • 200 ml (2 x 100 ml)
  • 500 ml (2 x 250 ml)
  • 1 l (2 x 500ml)
  • 325175
  • 325176
  • 325177
Chemiluminescent Elite Plus 18 months when stored at 4°C
  • High sensitivity, picogram (10-12 g) higher range
  • Luminol-based chemistry
  • Two component system
  • 200 ml (2 x 100 ml)
  • 500 ml (2 x 250 ml)
  • 1 l (2 x 500ml)
  • 324175
  • 324176
  • 324177
Chemiluminescent Elite 18 months when stored at 4°C
  • High sensitivity, picogram (10-12 g) lower range
  • Luminol-based chemistry
  • Two component system
  • 200 ml (2 x 100 ml)
  • 500 ml (2 x 250 ml)
  • 1 l (2 x 500ml)
  • 323175
  • 323176
  • 323177

Chemiluminescent Alkaline Phosphatase (AP) Microwell and Membrane

ProductShelf LifeProduct FeaturesAvailable SizesProduct Numbers
Chemiluminescent AP Select Plus 450 24 months when stored at 4°C
  • Most sensitive formula, attogram (10-18 g) higher range
  • Dioxetane-based chemistry
  • RTU (ready-to-use)
  • 200 ml
  • 500 ml
  • 1 l
  • 326175
  • 326176
  • 326177
Chemiluminescent AP Select Plus 540 24 months when stored at 4°C
  • Excellent sensitivity, attogram (10-18 g) higher range
  • Dioxetane-based chemistry
  • RTU (ready-to-use)
  • 200 ml
  • 500 ml
  • 1 l
  • 327175
  • 327176
  • 327177
Chemiluminescent AP Select 540 24 months when stored at 4°C
  • High sensitivity, attogram (10-18 g) lower range
  • Dioxetane-based chemistry
  • RTU (ready-to-use)
  • 200 ml
  • 500 ml
  • 1 l
  • 329175
  • 329176
  • 329177
Chemiluminescent AP Select 450 24 months when stored at 4°C
  • Least sensitive formula, attogram (10-18 g) lower range
  • Dioxetane-based chemistry
  • RTU (ready-to-use)
  • 200 ml
  • 500 ml
  • 1 l
  • 328175
  • 328176
  • 328177