TMB Membrane Substrates from Neogen® are ready-to-use, one-bottle solutions that offer sensitive horseradish peroxidase (HRP) detection for membrane assays. They are intended for western blot, dot blots and line assays and offer comparable performance & sensitivity to some chemiluminescent substrates. Our TMB (3,3',5,5'-tetramethylbenzidine) Membrane Substrates develop an insoluble, permanent dark blue reaction precipitate in the presence of horseradish peroxidase. These substrates are not recommended for microwell or immunohistochemical applications. Available Products TMB Membrane Substrate TMB Membrane Plus Substrate For research and manufacturing use only.