Neogen ELISA Drug Detection KitCortisol, or hydrocortisone, is the primary corticosteroid secreted by the adrenal cortex. Cortisol is synthesised from cholesterol and may be found in the blood as free Cortisol or bound to corticosteroid-binding globulin. The release of Cortisol is controlled by ACTH, which is produced in the anterior pituitary. Plasma Cortisol levels are highest in the morning and decrease throughout the day. Cortisol concentration in the plasma also elevates in response to stress. Cortisol has an anti-inflammatory effect and aids in carbohydrate metabolism, renal function and the promotion of glucogenesis.

Measurement of plasma Cortisol levels is useful in diagnosing conditions related to functions of the adrenal cortex, including Cushing's syndrome (hypercortisolism), Addison's disease (hypocortisolism) and adrenal tumours. Abnormal Cortisol levels may also possibly be linked to prostate cancer, depression, and schizophrenia.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Cortisol 100%
Prednisolone 47.4%
Cortisone 15.7%
11-Deoxycortisol 15%
Prednisone 7.83%
Corticosterone 4.81%
6β-Hydroxycortisol 1.37%
17-Hydroxyprogesterone 1.36%
Deoxycorticosterone 0.94%
Progesterone 0.06%
Bethamethasone 0.05%
Dehydroepiandrosterone 0.03%
Dexamethasone 0.03%
Beclomethasone 0.01%
d-Aldosterone 0.01%
Testosterone 0.01%
17α-Hydroxypregnenolone 0.01%
Androstenedione < 0.01%
Cholesterol < 0.01%
Estradiol < 0.01%
Estriol < 0.01%
Estrone < 0.01%
Pregnenolone < 0.01%


   80% B/B0 = 0.04 ng/mL
   50% B/B0 = 0.2 ng/mL
Assay Range: 0.04 - 10 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
402710 Cortisol Kit, 96 well kit

Reference Materials

402710 - Cortisol ELISA Kit, Kit Insert [English]
402710 - Cortisol ELISA Kit, Product Information Sheet [English]
402710 - Cortisol ELISA Kit, Safety Data Sheet [English]

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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