Activity of Neogen's TMB Substrates

Custom TMB Substrate is a one bottle stabilised chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) Custom TMB Substrate develops a deep blue colour in the presence of peroxidase labelled conjugate. This substrate has been optimised for use with microwell assays, especially ELISA. Custom TMB is not for use with assays requiring a precipitating substrate.

Sensitivity: Custom TMB Substrate is ideal for assays requiring a slower kinetic reaction. This formulation provides an activity level 20-30% lower than Neogen’s Enhanced K-Blue formulation. Custom TMB offers excellent signal-to-noise and has very low background.

Appearance: Clear to faint blue solution

Stability and Storage: Custom TMB Substrate is stable for a minimum of 36 months when stored at 4°C.

Custom Packaging Service: Custom TMB Substrate in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen representative.

Order Details

Product # Product Description
309175 Custom TMB Substrate, 200 mL
309176 Custom TMB Substrate, 500 mL
309177 Custom TMB Substrate, 1 L
309257 Custom TMB Substrate, 20 L (1 x 20 L)

For research and manufacturing use only.

Please check with your local representative for availability of these products within your region.