Enhanced K-Blue® is a one bottle stabilised chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) Enhanced K-Blue develops a deep blue colour in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimised for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Sensitivity: Enhanced K-Blue is a highly sensitive substrate with kinetic reactions equivalent to Neogen’s original K-Blue substrate formulation. This formulation offers a linear response across a wide range of HRP concentrations and has a rapid turn-over rate. Enhanced K-Blue also features high signal-to-noise and offers very low background. Stability and Storage: Enhanced K-Blue has the longest shelf life compared to any other TMB substrate on the market and is stable for a minimum of 48 months when stored at 4°C. Appearance: Clear to faint blue solution. Custom Packaging Service: Neogen can package Enhanced K-Blue in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen representative. Order Details Product # Product Description 308175 Enhanced K-Blue TMB Substrate, 200 mL 308176 Enhanced K-Blue TMB Substrate, 500 mL 308177 Enhanced K-Blue TMB Substrate, 1 L 308257 Enhanced K-Blue TMB Substrate, 20 L (1 x 20 L) Reference Materials 308175, 308176, 308177, 308257 - Enhanced K-Blue Substrate, Safety Data Sheet [English]308175, 308176, 308177, 308257 - Enhanced K-Blue Substrate, Safety Data Sheet [French] For research and manufacturing use only.