Activity of Neogen's TMB Substrates

Enhanced K-Blue® is a one bottle stabilised chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) Enhanced K-Blue develops a deep blue colour in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimised for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate.

Sensitivity: Enhanced K-Blue is a highly sensitive substrate with kinetic reactions equivalent to Neogen’s original K-Blue substrate formulation. This formulation offers a linear response across a wide range of HRP concentrations and has a rapid turn-over rate. Enhanced K-Blue also features high signal-to-noise and offers very low background.

Stability and Storage: Enhanced K-Blue has the longest shelf life compared to any other TMB substrate on the market and is stable for a minimum of 48 months when stored at 4°C.

Appearance: Clear to faint blue solution.

Custom Packaging Service: Neogen can package Enhanced K-Blue in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen representative.

Order Details

Product # Product Description
308175 Enhanced K-Blue TMB Substrate, 200 mL
308176 Enhanced K-Blue TMB Substrate, 500 mL
308177 Enhanced K-Blue TMB Substrate, 1 L
308257 Enhanced K-Blue TMB Substrate, 20 L (1 x 20 L)

Reference Materials

308175, 308176, 308177, 308257 - Enhanced K-Blue Substrate, Safety Data Sheet [English]
308175, 308176, 308177, 308257 - Enhanced K-Blue Substrate, Safety Data Sheet [French]

For research and manufacturing use only.

Please check with your local representative for availability of these products within your region.