Neogen's line of reagents include ready-to-use substrates and liquid reagents for use with horseradish peroxidase (HRP) and alkaline phosphatase (AP) based immunoassays. Neogen’s reagents are used worldwide by diagnostic test kit manufacturers and independent researchers. The substrates offer easy-to-use formats with excellent sensitivity, stability and lot-to-lot consistency that make them ideal for inclusion in commercial immunoassay test kits, or for individual in-house assay development projects. Neogen's substrates can be ordered in a range of bulk packaging or custom fill options. Speak with a Neogen representative to see how these options can benefit your business. Solutions Chemiluminescent AP Substrates Neogen offers ultra-sensitive 1,2-dioxetane-based chemiluminescent substrate formulations for detection of alkaline phosphatase in microwell and membrane applications. Chemiluminescent HRP Substrates Neogen offers luminol-based chemiluminescent substrate formulations for microwell peroxidase applications that meet a wide range of detection requirements. Colourimetric AP Substrates (Membrane) BCIP/NBT Membrane Substrate is a one-bottle, ready-to-use buffered alkaline phosphatase (AP) substrate. These substrates are not recommended for microwell or immunohistochemical applications. Colourimetric HRP Substrates (Membrane) TMB Membrane Substrate is a ready-to-use solution. TMB Membrane Substrate develops an insoluble, permanent dark blue reaction in the presence of peroxidase. This substrate is not recommended for microwell or immunohistochemical applications. Colourimetric AP Substrates (Microwell) K-Gold® Substrate is a one-bottle stabilised chromogenic substrate for use with alkaline phosphatase based immunoassays. K-Gold Substrate utilizes the substrate p-nitrophenyl-phosphate (PNPP), which reacts with alkaline phosphatase to produce the chromophore p-nitrophenol. Neogen’s ready-to-use K-Gold formula does not require mixing of additional ingredients or stabilizing agents, making it an ideal formula for kit manufacturers. Colourimetric HRP Substrates (Microwell) Neogen recognises that one TMB microwell substrate formulation will not meet the specifications of all HRP-based immunoassays. Therefore, Neogen offers multiple unique one-bottle TMB microwell colorimetric substrate formulations to meet specific requirements for different assay systems. ELISA Buffers Neogen’s colourimetric microwell reagents are designed for use with ELISA kits. We recognise that additional reagents may be required for your assay applications. Therefore, we make available the following products that are optimised with our ELISA kits. Neogen’s reagents can also be used with other manufacturer’s ELISA kits. Stop Solution Neogen’s Red Stop Solution is a non-acidic, ready-to-use ELISA stop solution that contains no hazardous acids and can be used with our TMB substrates. Red Stop Solution does not increase background or optical density (OD) readings. Absorbance readings are taken at 650 nm within 2 hours of adding Red Stop Solution to the assay wells. Red Stop Solution offers a safer alternative to the traditional acids that are used to stop ELISA reactions. Custom Fill Packaging Allow us to package substrates and reagents in custom bottle sizes and volume fills to meet your specific packaging requirements.