Neogen ELISA Drug Detection KitTestosterone is the principle androgen. It is synthesised in the testis, the ovary and the adrenal cortex. It is responsible for the development and maintenance of the male secondary sex characteristics. It also exerts important protein anabolic and growth-promoting effects. The plasma testosterone levels are useful in investigating hypogonadism and hormone replacement therapy in men. It is also useful as a marker in hyper-androgenism in women.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Testosterone 100%
Dihydrotestosterone 100%
Androstenedione 0.86%
Bolandiol 0.86%
Testosterone Enanthate 0.13%
Estriol 0.10%
Testosterone Benzoate 0.10%
Estradiol 0.05%
Dehydroepiandrosterone 0.04%
Testosterone Propionate 0.04%
Deoxycorticosterone 0.03%
Testosterone 17β-Cypionate 0.02%
Aldosterone < 0.01%
Corticosterone < 0.01%
Cortisol < 0.01%
Cortisone < 0.01%
Estrone < 0.01%
17-Hydroxyprogesterone < 0.01%
Pregnenolone < 0.01%
Progesterone < 0.01%


   80% B/B0 = 0.006 ng/mL
   50% B/B0 = 0.029 ng/mL
Assay Range: 0.002 - 0.2 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
402510 Testosterone Kit, 96 well kit

Reference Materials

402510 - Testosterone ELISA Kit, Kit Insert [English]
402510 - Testosterone ELISA Kit, Product Information Sheet [English]
402510 - Testosterone ELISA Kit, Safety Data Sheet [English]

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

Neogen has partnered with to provide an online tool for data calculations.

Follow the simple step-by-step guide to generate your data.

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For research use only.

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