TMB Membrane Plus Substrate is a ready to use solution. It contains 3,3’,5,5’-tetramethyl-benzidine in a mildly acidic buffer in a one bottle format. Unreacted substrate should be faint yellow to pink in appearance. TMB Membrane Plus Substrate develops an insoluble, permanent dark blue reaction in the presence of peroxidase. This substrate is not recommended for microwell or immunohistochemical applications. It is intended for western blot, dot blots and line assays.

Sensitivity: TMB Membrane Plus Substrate is extremely sensitive. Offering HRP detection in the low picogram range, it can rival some chemiluminescent substrates for HRP detection. This formula also offers an extended dynamic range making it resistant to wash-out at higher HRP concentrations. TMB Membrane Plus also features a high signal-to-noise ratio, making it resistant to fading.

Stability and Storage: TMB Membrane Plus Substrate is stable for 2 years, from the date manufacture, when stored at room temperature (15° to 25°C) or 48 months when stored at 2° to 8°C.

Custom Packaging Service: Neogen can package TMB Membrane Plus Substrate in custom bottle sizes and volume fills to meet your specific packaging requirements. This service is a time and cost saving feature for any test kit manufacturer. For details on this service, please contact a Neogen Corporation representative.

Order Details

Product # Product Description
337247 TMB Membrane Plus Substrate, 100 mL
337177 TMB Membrane Plus Substrate, 1 L

For research and manufacturing use only.

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